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KMID : 0360220110520091089
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 9 p.1089 ~ p.1093
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Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells
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Lee Jeong-Hun
Kim Jae-Woo
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Abstract
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Purpose:To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC).
Methods:Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay, respectively.
Results:Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant.
Conclusions:Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow.
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KEYWORD
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Atropine, Cyclopentolate, Pilocarpine, Trabecular meshwork cells, Tropicamide
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